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Thanks for your advice, we'll improve this in future videos.

Our pre-coated ELISA kits contain a complete set of reagents and detailed operating manual for ELISA experiments, which do not require additional reagents and buffers to be purchased.

It is not very suitable. If cells can be dissociated from a three-dimensional matrix to form a single-cell suspension, then it is possible to consider making single-cell suspension into a cell smear sample and using this reagent kit. Alternatively, after obtaining single-cell suspension, flow cytometry can be performed using a flow cytometry channel kit.

Thanks~

You can use any drawing software that has the function of fitting curves.If the effect of the drug or intervention is to inhibit cell viability, and we usually want to get the IC50, then our abscissa will be the specific drug concentration, and the ordinate will be 100% minus the growth rate in the video. For example, we can assume that the concentrations (mM) of NC and Test1~4 groups are 0, 1000, 100, 10, 1 respectively, then the data information we get is shown in the figure：(I'm sorry that we can't send picture in the reply. If you need the graph, could you offer us your email address, then we can send it to your mailbox.) Then they are entered into the software, the pictures and formulas are obtained, and the IC50 can be calculated using the software, the following is the diagram with “CurveExpert1.4” as an example. Right-click on the graph to select “Analysis”, and the IC50 can be calculated.

It depends on your grouping settings, and the following information is for your reference: NC in CCK-8 experiments usually refers to normal cells that have not been treated, although if you are dealing with something like lipid transfection, NC usually refers to the group that has been transfected with NC fragments.

The oven is sufficient, the incubator is better, and when the incubator is applied, pay attention to separating it from other cells cultured to prevent contamination, for example, you can use a separate incubator;As shown in the video, the sample is first placed in a humidity box protected from light, where humidity is already guaranteed, and the purpose of this step is to prevent the liquid that infiltrates the sample from evaporating completely, so there is usually no need to increase the humidity.For some samples, if an extended incubation time is required, it can be observed every 30 minutes to ensure that the sample remains immersed in the liquid at the end of the incubation time.

Yes. However, it is worth noting that cell slide are usually more suitable for cell lines or primary cells, and for tissue samples, the disadvantage is that the process of preparing tissue into a single-cell suspension may introduce damage or other interventions, so perhaps a video of the processing of tissue samples is more appropriate, here are the related links: Paraffin Section： www.elabscience.com/resource-cell_function-detail-1032 Frozen Section： www.elabscience.com/resource-cell_function-detail-1036

In general, for the accuracy of the test results, it is recommended that both the standard and the sample be dupicate wells.

If there is no problem with your operation, and the number of samples is relatively large, you can choose standard to do a single well.

You're right. Each group needs to change the tip, and we actually do. It's just to shorten the video duration, so we didn't show this detail in the video.

We have not verified pollen samples, but the use of anti-stripping slides can reduce the incidence of stripping.

It means the group whose cells have not been treated with drugs.

thanks@@Elabscience_Bio

Yes, we use the same bottle of standard & sample diluent that comes with the kit.

Clinical sensitivity of UNCOV-40 is 98.81%(95% CI *: 97.25%, 99.61%), specificity is 98.02% (95% CI*: 97.05%, 98.74%). ''Up to" means this kits has high clinical specificity and sensitivity, not the maximum performance value.

Hi ShriSJ, different supplier has their own standard, it's better to confirm with them.

Please leave your message on our website contact page: www.elabscience.com/contact_us-88.html Then we will try our best to reply you, generally less than 12hours.

Please provide details of the specific color and shape of the dot, or preferably a picture for more accurate analysis.

no

@@Elabscience_Bio so if u have common colds or flu or hiv, it wont be detected if u undergo a test using this testing kit for covid 19?

The specification of the kit is 40T and contains 40 test cassettes, which can be used for 40 samples' detection. However, each test cassette should be used within 1h after opening and cannot be reused.

For more accurate analysis, it will be highly appreciated if you could share the criteria of cure and IgG detection result in advance.

yes, ELISA kit operation guide.

Thanks~